I'd include anything that has been published . . . letters to the editor, newspaper clippings (high school, college, or whatever you might have), magazine articles, and website copy (other than Facebook, *grin*)
Papers or essays that you are especially proud of (especially if you haven't been published much and they fit the style of the writing you hope to do). I wouldn't go too heavy here. Editors do not enjoy reading ten-page missives on
The Scarlett Letter. Most local media doesn't publish poetry or short stories—I'd skip those (unless they have been published).
Possibly a sample article in the style that you would want to write in the job you are applying for. A current subject would be best. If you want to be a sports writer, for example, I'd include a sample piece on a recent game. Some publications will assign you a sample article to see your take on things and your ability to turn things on a deadline.
A brief resume that can fit onto one page. Highlight any professional writing (paid or volunteer), writing/editing experience, and advanced coursework that can establish you as a trusted writer.
A cover letter stating what position you are applying for and what makes you a good candidate for that position.
If your portfolio is a little weak in some areas (nothing published to date), you could include letters of reference as well.
No, I didn't come up with all of this on my own. I asked a professional editor.
Eric Noto